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. 2023 Aug 15;4(3):102518. doi: 10.1016/j.xpro.2023.102518

Figure 2.

Figure 2

Design of HDR template

(A) Illustration depicting the binding of Cas9-crRNA-tracrRNA complex with the target DNA.

(B) Example of asymmetric donor DNA design with the target strand, resulting in a high HDR rate.