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Expected outcomes of a 3×Flag tag knock-in project
(A) Expression of the GM4951 protein with a 3×Flag tag in several tissues from two different founders. The highest expression of the GM4951 protein was observed in the liver, which is consistent with the expression profile of the Gm4951 mRNA.
(B) Genotyping results of 3×Flag tag Gm4951 knock-in mice after breeding for two generations. Two sets of genotyping primers (FI + RI, FT + RT) were used.
