Reagent | Amount |
---|---|
Forward oligo (10 μM) | 1 μL |
Reverse oligo (10 μM) | 1 μL |
2× JumpStart Taq ReadyMix | 12.5 μL |
Crude genomic DNA | 0.5 μL |
ddH2O | to 25 μL |
Reagent | Amount |
---|---|
Forward oligo (10 μM) | 1 μL |
Reverse oligo (10 μM) | 1 μL |
2× JumpStart Taq ReadyMix | 12.5 μL |
Crude genomic DNA | 0.5 μL |
ddH2O | to 25 μL |