Temperature | Time | |
---|---|---|
95° C | 2 min | |
95° C | 30 s | 35 cycles |
55° C–68° C (Average Tm of primers - 5° C) | 30 s | |
72° C | 30 s | |
72° C | 5 min | |
4° C | Hold |
Temperature | Time | |
---|---|---|
95° C | 2 min | |
95° C | 30 s | 35 cycles |
55° C–68° C (Average Tm of primers - 5° C) | 30 s | |
72° C | 30 s | |
72° C | 5 min | |
4° C | Hold |