Skip to main content
. 2023 Aug 15;4(3):102518. doi: 10.1016/j.xpro.2023.102518
Genotyping PCR program
Temperature Time
95° C 2 min
95° C 30 s 35 cycles
55° C–68° C (Average Tm of primers - 5° C) 30 s
72° C 30 s
72° C 5 min
4° C Hold