REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-FLAG M2 antibody, 1:5000 dilution	Sigma-Aldrich	F3165
Rabbit anti-GAPDH (D16H11) antibody, 1:1000 dilution	Cell Signaling Technology	5174
Peroxidase AffiniPure Goat Anti-Mouse IgG, Light Chain Specific, 1:5000 dilution	Jackson ImmunoResearch	115-035-174
Peroxidase AffiniPure Goat Anti-Rabbit IgG (H + L), 1:5000 dilution	Jackson ImmunoResearch	111-035-144
Bacterial and virus strains
Stellar Competent Cells	Clontech	636763
Chemicals, peptides, and recombinant proteins
EMEM	ATCC	30–2003
FBS	ATCC	30–2020
Penicillin-Streptomycin 100×	Gibco	15-140-122
1 M Tris-Cl pH 8.3	Teknova	T1083
KCl	Sigma-Aldrich	P9541
MgCl2.6H2O	Sigma-Aldrich	M2393
IGEPAL	Sigma-Aldrich	I8896
Tween 20	Sigma-Aldrich	P9416
Gelatin	Sigma-Aldrich	G9391
1 M Tris-Cl pH 7.6	Teknova	T1076
NaCl	Sigma-Aldrich	S3014
Sodium deoxycholate	Sigma-Aldrich	30970
SDS	Sigma-Aldrich	L3771
10× CutSmart Buffer	NEB	B7204
BbsI-HF	NEB	R3539S
10× T4 Ligase Buffer	NEB	B0202S
Lipofectamine 2000	Invitrogen	11668019
Opti-MEM	Gibco	31985070
Proteinase K	Roche	3115879001
JumpStart Taq ReadyMix	Sigma-Aldrich	P0982
10× NEBuffer 2	NEB	B7002S
T7 endonuclease	NEB	M0302S
Protease inhibitor	Roche	11873580001
4%–12% NuPAGE Bris-Tris gel	Invitrogen	NP0336BOX
20× MOPS buffer	Invitrogen	NP001
Nitrocellulose Transfer Kit	Bio-Rad	1704270
10× TBST	Teknova	T9511
Critical commercial assays
QIAquick Gel Extraction Kit	QIAGEN	28706
QIAprep Spin Miniprep Kit	QIAGEN	27106
Experimental models: Cell lines
Neuro-2a cells	ATCC	CCL-131
Experimental models: Organisms/strains
C57BL/6J mice (4–6 weeks old, male and female)	The Jackson Laboratory	000664
Oligonucleotides
Primers	IDT	N/A
ssDNA oligos	IDT	N/A
crRNA	IDT	N/A
Recombinant DNA
PX458	Addgene	48138
PX459	Addgene	62988