Reagent | Final concentration | Amount |
---|---|---|
1 M Tris-Cl pH 7.6 | 25 mM | 12.5 mL |
5 M NaCl | 150 mM | 15 mL |
IGEPAL | 1% v/v | 5 mL |
Sodium deoxycholate | 1% w/v | 5 g |
10% SDS | 0.1% w/v | 5 mL |
Total | N/A | 500 mL |
Reagent | Final concentration | Amount |
---|---|---|
1 M Tris-Cl pH 7.6 | 25 mM | 12.5 mL |
5 M NaCl | 150 mM | 15 mL |
IGEPAL | 1% v/v | 5 mL |
Sodium deoxycholate | 1% w/v | 5 g |
10% SDS | 0.1% w/v | 5 mL |
Total | N/A | 500 mL |