DNA ligation mix
| Reagent | Amount |
|---|---|
| Digested vector (50 ng/μL) | 1 μL |
| Annealed oilgos (1:200 dilution in ddH2O) | 1 μL |
| 10× T4 Ligase Buffer (NEB) | 1 μL |
| T4 DNA Ligase | 0.5 μL |
| ddH2O | To 10 μL |
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