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. 2023 Aug 18;12(8):e12345. doi: 10.1002/jev2.12345

FIGURE 2.

FIGURE 2

Macrophage EVs modulate cellular apoE protein levels and the phagocytic capacity of recipient macrophages. (a‐b) Western blot analysis (a) and quantification (b) of ApoE protein levels in cell lysates of wildtype BMDM exposed to 2 × 109 particles of EKO‐BMDM‐EV, WT‐BMDM‐EV, or PBS for 18 h. (c,d) Representative histogram (c) and quantitative graph (d) showing MFI of CFSE‐labeled apoptotic Jurkat cells uptake in Apoe −/− BMDM or wildtype BMDM exposed to 2 × 109 particles of EKO‐BMDM‐EV, WT‐BMDM‐EV, or PBS for 18 h measured by flow cytometry. (e,f) Representative histogram (e) and quantitative graph (f) showing MFI of MERTK surface expression in Apoe −/− BMDM or wildtype BMDM exposed to 2 × 109 particles of EKO‐BMDM‐EV, WT‐BMDM‐EV, or PBS for 18 h measured by flow cytometry. One representative experiment out of two independent replicates is shown for all experiments; n = 4 per group. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 as determined using one‐way ANOVA followed by Holm‐Sidak post‐test. Data are presented as mean ± SEM.