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. 2023 Apr 7;164(9):1965–1975. doi: 10.1097/j.pain.0000000000002892

Figure 3.

Figure 3.

EA treatment increased proportions of GRAs and MONs and decreased the proportions of β-END+ CD11b+ and β-END+ ICAM-1+ cells on day 6 after CFA injection. (A) Flow cytometry plots showed the gating strategy for β-END+ cells in GRAs and MONs. (B) Bar graph showed the change of GRAs and MONs (% of total) on day 6 after CFA injection in 3 groups (n = 9). (C and D) Flow cytometry plots and bar graph show the expression of β-END in GRAs and MONs in 3 groups (n = 9). (E) Gating strategy of β-END+ ICAM-1+ cells and β-END+ CD11b+ cells in GRAs and MONs. Flow cytometry plots and bar graphs show the proportions of β-END+ CD11b+ (F and G) and β-END+ ICAM-1+ (H and I) cells in GRAs and MONs in 3 groups. **P < 0.01 vs control group, #P < 0.05 vs CFA group. All data are shown as mean ± SEM; statistical analyses were performed using 1-way ANOVA followed by the Tukey HSD test. ANOVA, analysis of variance; β-END, β-endorphins; CFA, complete Frester adjuvant; EA, electroacupuncture; FSC, forward scatter; FSC-A, forward scatter area; GRAs, granulocytes; MONs, monocytes; SSC, side scatter; SSC-A, side scatter area; SSC-H, side scatter height.