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. 2023 Apr 7;164(9):1965–1975. doi: 10.1097/j.pain.0000000000002892

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Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the International Association for the Study of Pain.

This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

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Figure 5. — RNA-sequencing analysis and qRT-PCR verification of genes related to cell migration. (A) Heat map of cell migration–associated genes. Different heat map colors represent the relative mRNA expression levels of genes calculated by Log₂ (FPKM + 1). (B) Violin plots of expression values for differentially expressed genes between CFA and EA groups. Results are expressed as FPKM. Data shown are the mean FPKM ± SEM (n = 5). *P < 0.05 vs CFA group. (C) Significant differentially expressed genes as determined by real-time PCR (n = 8). (D) Correlation between NE and the gene expression of Cxcl1 in inflammatory tissues based on the Pearson correlation analysis. All data are expressed as the mean ± SEM. *P < 0.05 vs CFA group. CFA, complete Frester adjuvant; EA, electroacupuncture; FPKM, fragments per kb of transcript per million fragments mapped; NE, norepinephrine.