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. 2023 Aug 18;19:126. doi: 10.1186/s12917-023-03672-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Overview of image of screening of transposon mutants with SYTO 9-stained biofilms. (A) plastic beads as internal reference, (B) wild-type strain IMT-5155 forming biofilm, (C) transposon mutant with down-regulated biofilm formation, (D) transposon mutant depicting slightly up-regulated biofilm formation, (E) transposon mutant exhibiting up-regulated biofilm formation and (F) transposon mutant with highly up-regulated biofilm