Fig. 3.

Biofilm formation. APEC wild-type strain IMT5155 (purple bar), selected transposon mutants (Mutant, red bar), and transposon mutants transformed with either empty vector pACYC177 (empty, blue bar) or pACYC177 plasmids bearing wild-type versions of transposon mutated genes (Comp, green bar) in M9 minimal media at 37 °C after 72 h. The data shown are mean values and standard deviation of three separate experiments in six wells replicate for each experiment. Wells not incubated with bacteria were used as a negative control