Table 2.
List of primers used in the study
| Gene name/Target Sequence | Primer Name | Sequence 5’to 3’ | |
|---|---|---|---|
| Primers for arbitrary PCR | |||
| Flanking sequence Tn5 | P9 | CGCAGGGCTTTATTGATT | |
| Arbitrary | Arbi5 | GGCCACGCGTCGACTAGTAC(N)10TACNG | |
| Flanking sequence Tn5 | P6 | CCTAGGCGGCCAGATCTGAT | |
| Arbitrary | Arbi2 | GGCCACGCGTCGACTAGTAC | |
| Primers for amplification of genes from wild-type APEC | |||
| nhaA | A10kFwd | ACATAAGCTTTTGACAATTAATCATCGGCTCG TATAATGTGTGGAGGAGGACAGCTATGAAAC ATCTGCATCGATTCTTTAGC | |
| A10kRev | ACATAAGCTTTCAAACTGATGGACGCAAACGA ACGCGTAACCAGC | ||
| fdeC | A7rFwd | ACATGGATCCTTGACAATTAATCATCGGCTCG TATAATGTGTGGAGGAGGACAGCTATGTCACA TTATAAAACAGGTC | |
| A7rRev | ACATGGATCCCTATTGCTGGGTAAGAGGC | ||
| yjhB | C4sFwd | ACATGGATCCTTGACAATTAATCATCGGCTCG TATAATGTGTGGAGGAGGACAGCTATGGCAAC AGCATGGTATAAAC | |
| C4sRev | ACATGGATCCTCATTTAGCCACGGATAG | ||
| lysU | D11rFwd | ACATGGATCCTTGACAATTAATCATCGGCTCGTATAATGTGTGGAGGAGGACAGCTATGTCTGAACAAGAAACACGGGG | |
| D11rRev | ACATGGATCCTTATTTCTGTGGGCGCATCG | ||
| ecpR | D2qFwd | ACATGGATCCTTGACAATTAATCATCGGCTCGTATAATGTGTGGAGGAGGACAGCTATGGAATG TCAAAACCGTTCTG | |
| D2qRev | ACATGGATCCTTACTGAACCAACTTATATATTTTTGAGTACAGC | ||
| AJB35136 | E4rFwd | ACATGGATCCTTGACAATTAATCATCGGCTCGTATAATGTGTGGAGGAGGACAGCTATGGAAG AAAATAATATGAAGACG | |
| E4rRev | ACATGGATCCTTAATAAATAGATTCATACATA GC | ||
| fdtA | H6nFwd | ACATGGATCCTTGACAATTAATCATCGGCTCG TATAATGTGTGGAGGAGGACAGCTATGGATAT TAAATTAATCTCTTTGC | |
| H6nRev | ACATGGATCCTTATGAATTCTCAATTGAATTTA CTCTTC | ||
| Insert sequencing after cloning | pACYC177Fwd | GTAGCGGTTCGGTTTATTGAC | |
| pACYC177Rev | TGTCCACGGTACGCCTGC | ||