Fig. 1.

Identification and characterization of hsa_circ_0067842 in BC. A Volcano plot showing the expression profiles of circRNAs. B Schematic illustrations displaying the genomic loci of hsa_circ_0067842, produced by exons 12 to 17 of the SMC4 gene. C-D ISH analysis detecting hsa_circ_0067842 expression (red) in BC tissue (n = 126) and adjacent normal tissues (n = 59). Representative images from C two BC cases and adjacent normal tissues are shown (magnification: ×200). E-F Kaplan–Meier analysis of the correlation between hsa_circ_0067842 levels and the disease-free survival or overall survival rate of BC patients. G Back-splice junction of hsa_circ_0067842 verified by Sanger sequencing. H Convergent or divergent primers were used to validate the existence of hsa_circ_0067842 in BC cells. I Total RNAs were treated with RNase R and qRT-PCR was used to detect hsa_circ_0067842 and SMC4. J The expression of hsa_circ_0067842 and SMC4 mRNA in BC cells treated with Actinomycin D at the indicated time points was detected by qRT-PCR. K FISH assay identifying the subcellular location of hsa_circ_0067842 in BC cell lines. Scale bar, 20 μm. Quantitative data were presented as the mean ± SD, ^***p < 0.001 and ^****p < 0.0001