Fig. 2.

Hsa_circ_0067842 promotes BC metastasis both in vitro. A qRT-PCR determining the expression of hsa_circ_0067842 in the normal breast epithelium cell line (MCF-10 A) and BC cell lines (normalized to β-actin). B-C qRT-PCR analysis of hsa_circ_0067842 expression in NC plasmid-, hsa_circ_0067842 plasmid-, si-NC-, si-hsa_circ_0067842-1-, and si-hsa_circ_0067842-2-treated BC cells. D-E Transwell migration and invasion assays assessing the changes in the migratory and invasive capabilities of MDA-MB-468 and MDA-MB-231 cells after transfection with si-hsa_circ_0067842s. F-G Transwell migration and invasion assays assessing the changes in the migratory and invasive capabilities of BT-549 and MCF-7 cells after transfection with NC or hsa_circ_0067842 plasmids. The data are assessed as the mean ± SD, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 and ^****p < 0.0001