Fig. 5.

Hsa_circ_0067842 increases mRNA stability of CMTM6 by regulating HuR and CMTM6 promotes migration and invasion of BC cells. A-B The expression of CMTM6 after knocking down HuR was verified by qRT-PCR and western blot. C RNA stability was evaluated using Actinomycin D in MCF-7 cell, and the degradation rates of the CMTM6 mRNA were measured at appointed time. D RNA immunoprecipitation experiments were performed in MDA-MB-468 and MDA-MB-231 cells and the coprecipitated RNA was subjected to qRT-PCR for CMTM6. E-H The expression of CMTM6 after altering hsa_circ_0067842 was verified by qRT-PCR and western blot. I RNA stability was evaluated using Actinomycin D in MDA-MB-468, and the degradation rates of the CMTM6 mRNA were measured at appointed time. J-K Transwell migration and invasion assays assessing the changes in the migratory and invasive capabilities of MCF-7 and BT-549 cells after transfection with si-CMTM6s. Values are shown as the mean ± SD of the mean from three independent experiments. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001