Skip to main content
. Author manuscript; available in PMC: 2023 Aug 18.
Published in final edited form as: ACS Chem Biol. 2021 Nov 15;16(12):2808–2815. doi: 10.1021/acschembio.1c00693

Figure 2.

Figure 2.

PhosTACs mediate ternary complex formation between Halo-PDCD4 and FKBP12(F36V)-PP2A A. (a) PhosTACs form a stable ternary complex with Halo-PDCD4 and FKBP12(F36V)-PP2A A in a linker length dependent manner. Halo-PDCD4/FKBP12(F36V)-PP2A A wild type HeLa cells were treated with indicated PhosTACs at a concentration of 5 μM for 24h, lysed for Halotrap pulldown, and analyzed by Western blot using indicated antibodies. (b) Inactive PhosTAC7F does not form ternary complex with Halo-PDCD4 and Halo-PP2A A. Halo-PDCD4/FKBP12(F36V)-PP2A A wild type or P179R mutant HeLa cells treated with indicated PhosTACs at a concentration of 5 μM for 24h, lysed for Halotrap pulldown, and analyzed by Western blot using indicated antibodies. (L.E.: long exposure; S.E.: short exposure) (c) PhosTAC7 efficiently recruits PP2A A and C subunits to Halo-PDCD4. Halo-PDCD4/FKBP12(F36V)-PP2A A wild type HeLa cells treated with indicated PhosTACs at a concentration of 5 μM for 24h, lysed for Halotrap pulldown, and analyzed by Western blot using indicated antibodies.