Table 2. Number of chlamydial DNA positive (qPCR) and cell culture swabs before and after decontamination procedures.
| Site sampled | Sampling events | DNA positive (qPCR) | Cell culture | ||
|---|---|---|---|---|---|
| Examination room | Before | After | Before | After | |
| Examination bench | 16 | 1 | 1 | – | – |
| Ultrasound probe | 16 | 1 | 2 | + | – |
| Stethoscope | 18 | 2 | 1 | – | – |
| Oxygen mask | 18 | 4 | 5b | +/++/−/− | +/−/−/−/NT |
| Recovery basket | 10 | 0 | 0 | NT | NT |
| Capture bag | 4 | 1 | 0 | +++ | NT |
| Personnel | |||||
| Clinicians’ shoes | 8 | 0 | 0 | NT | NT |
| Clinicians’ shirt | 8 | 3 | 0 | ++ | NT |
| Clinicians’ hands | 4 | 1 | 0 | – | NT |
| Volunteer shoes | 14 | 0 | 0 | NT | NT |
| Volunteer hands | 22 | 2 | 1 | – | ++ |
| Enclosures | |||||
| Door handle | 8 | 0 | 0 | NT | NT |
| Walls | 30 | 2a | 0 | +++/++ | NT |
| Mesh | 8 | 0 | 0 | NT | NT |
| Floor | 10 | 1 | 0 | – | NT |
| PVC browse holder | 10 | 1 | 0 | +++ | NT |
| Towel under koala with urogenital disease (bleach wash) | 8 | 0 | 0 | NT | NT |
| Towel under koala with urogenital disease (regular wash) | 8 | 0 | 0 | NT | NT |
| Timber perches | 19 | 0 | 1 | NT | – |
Note:
NT, not tested; +, very few viable chlamydial organisms detected; ++, moderate numbers of viable chlamydial organisms detected; +++, many viable chlamydial organisms detected; −, no viable chlamydial organisms detected. aone sample in enclosure of infected koala, one adjacent. bno “before” sample available for one positive “after” sample.