FIG. 6.

Overexpression of DNA2 leads to nongrowing cells. WT, nontransformed MW35a cells grown on SC media; WT+pGAL-vector, MW35a cells containing the empty vector and grown on SC-Ura plates; WT+pGAL-DNA2, MW35a cells transformed with a plasmid that contained the DNA2 gene under the control of a galactose-inducible promoter (see Materials and Methods for construction of the plasmids). The plates contained glucose (left) or galactose (right) as a carbon source.