FIG. 7.

Induction of DNA2 expression leads to a transient increase of single-stranded telomeric G-rich DNA. MW35a cells containing pGAL-DNA2 were pregrown in SC-Ura media containing glycerol and lactate. Expression of DNA2 was then induced by the addition of galactose, and DNA was prepared from the culture at the indicated times after galactose addition. These DNAs were then digested with XhoI and analyzed by nondenaturing in-gel hybridization (left) using an end-labeled CA oligonucleotide as a probe. After denaturation of the DNA, the gel was rehybridized to the same probe as a control (right). Controls and molecular size standards are as in Fig. 2 through 5.