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. 1999 Jun;19(6):4182–4190. doi: 10.1128/mcb.19.6.4182

FIG. 3.

FIG. 3

Id2 and γ-globin are up-regulated in OSI-2040-induced K562 cells. (A) Southern blot of the cDNA pools and amplicons shown in Fig. 2A obtained with an Id2 probe. Lanes − and +, cDNA from uninduced and OSI-2040-induced K562 cells. Lanes F1 to F3 represent successive rounds of subtraction in the forward direction with induced K562 cDNA as the tester and uninduced K562 cDNA as the driver. Lanes R1 and R2 represent successive rounds of subtraction in the reverse direction with uninduced K562 cDNA as the tester and induced K562 cDNA as the driver. (B) Northern analysis of OSI-2040-induced K562 cells. Two milligrams of poly(A)+ RNA from uninduced (−) or OSI-2040-induced (+) K562 cells was analyzed with γ-gene and Id2 probes. S14 served as the control. (C) Northern analysis of OSI-2040-induced K562 cells. Two milligrams of poly(A)+ RNA from uninduced (−) or OSI-2040-induced (+) K562 cells was analyzed with ɛ-gene, β-gene, and α-gene probes. S14 served as the control.