Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Jun;19(6):4182–4190. doi: 10.1128/mcb.19.6.4182

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 1999, American Society for Microbiology

PMC Copyright notice

FIG. 6 — An E box in HS2 mediates Id2 induction. (A) Diagrammatic representation of the constructs used for transfection into Id2⁺ and Id2⁻ K562 cells. Wild-type HS2 contains the HindIII-XbaI fragment of HS2 linked to the −260 γ-promoter–luciferase gene hybrid. Conserved and protein binding sites within HS2 are labelled. Mutated sites are denoted by solid black circles. (B) Fold induction of the constructs depicted in panel A. The fold increase in luciferase levels between Id2⁻ and Id2⁺ K562 cells is shown for each construct. Luciferase values were corrected for the protein concentration of the lysate. Values are the means of at least three separate transfections of more than 50 pools with at least two different plasmid preparations. (C) Binding of proteins to the 8762 E box. EMSA was conducted with nuclear extracts from Id2⁻ (lanes 1 and 3) and Id2⁺ (lane 2) K562 cells. A USF probe, used as an unlabelled competitor, was added to lane 3 in 50-fold molar excess.

FIG. 6 — An E box in HS2 mediates Id2 induction. (A) Diagrammatic representation of the constructs used for transfection into Id2⁺ and Id2⁻ K562 cells. Wild-type HS2 contains the HindIII-XbaI fragment of HS2 linked to the −260 γ-promoter–luciferase gene hybrid. Conserved and protein binding sites within HS2 are labelled. Mutated sites are denoted by solid black circles. (B) Fold induction of the constructs depicted in panel A. The fold increase in luciferase levels between Id2⁻ and Id2⁺ K562 cells is shown for each construct. Luciferase values were corrected for the protein concentration of the lysate. Values are the means of at least three separate transfections of more than 50 pools with at least two different plasmid preparations. (C) Binding of proteins to the 8762 E box. EMSA was conducted with nuclear extracts from Id2⁻ (lanes 1 and 3) and Id2⁺ (lane 2) K562 cells. A USF probe, used as an unlabelled competitor, was added to lane 3 in 50-fold molar excess.