Skip to main content
. 2023 Aug 18;18(8):e0290340. doi: 10.1371/journal.pone.0290340

Fig 1. AKT1 interacts specifically with DNAJB3.

Fig 1

(a) SDS-PAGE analysis of the recombinant GST-DNAJB3 and GST control proteins expressed and purified from E. coli. Different fractions of the elutes were resolved by SDS-PAGE and stained with Coomassie blue. (b) GST-pull down experiments were carried out using GST-DNAJB3 recombinant protein or GST (a negative control) and whole cell lysate prepared from C2C12 cells. After binding, the resin was washed extensively with the binding buffer containing increasing concentrations (0.1–1 M) of NaCl. Bound proteins were eluted with 20 mM GSH followed by 1% SDS, resolved by SDS-PAGE and stained with silver nitrate. Proteins present in the 1 M NaCl wash were loaded in parallel. The binding of GST and GST-DNAJB3 without lysate was also included. The protein bands that interact specifically with GST-DNAJB3 are indicated by a red dot. As similar pattern was also obeseved in 3T3-L1 adipocytes and HepG2 cells (S1 Fig in S1 File). Splice points are shown as vertical arrows.