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. 2023 Aug 18;18(8):e0290340. doi: 10.1371/journal.pone.0290340

Fig 6. AKT Knockdown and DNAJB3 overexpression transfection efficiency in C2C12 cells.

Fig 6

Knocking down the expression of AKT with 20 nM of specific siRNA blunted the endogenous expression of AKT mRNAs. Actin was used as internal control for RT-PCR (upper panel). Transient overexpression of DNAJB3 in C2C12 cells (lower panel). All assays were performed at least in triplicate and a minimum of three independent experiments. Results are presented as means ± SEM and were plotted using GraphPad (Prism v7, La Jolla, CA). Shapiro-Wilk test was first performed for the normality test followed by parametric tests.