FIG. 5.

Effects of various inhibitors on ERK activation in ΔRaf-1:ER and PC12 cells. (A) ΔRaf-1:ER cells were either untreated (CTRL) or were pretreated with 400 nM PDBu for 24 h, 1 μM Gö 6976 (Go) for 2 h, 5 μM rottlerin (Rott) for 6 h, or 1 μM chelerythrine chloride (CC) for 2 h and then stimulated with (+) or without (−) 1 μM estradiol for 30 min. After cell lysis, equal protein aliquots were resolved by SDS-PAGE (10% polyacrylamide) and immunoblotted with anti-phospho-ERK antibody. (B) PC12 cells were either untreated (CTRL) or were pretreated with 400 nM PDBu (PDBu) for 24 h or 5 μM rottlerin (Rott) for 6 h and then stimulated with 10 ng of EGF, FGF, or NGF per ml for 10 min. Samples were then processed as for panel A.