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. 2023 Aug 18;9(33):eadg8631. doi: 10.1126/sciadv.adg8631

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Fig. 4. — (A) Schematic showing the reaction of the original PMSA. This PMSA used PEG₄₈-MAL that selectively attacks polysulfides alkylated by IAM at proximal sulfur atoms. Polysulfide-rich proteins thus acquire PEGylation and demonstrate low gel mobility. (B) Detection of supersulfidation of recombinant WT hADH5 before and after GSNOR and FDH reactions by using the original PMSA.