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. 2023 Aug 18;9(33):eadh3635. doi: 10.1126/sciadv.adh3635

Fig. 5. UFBP1 is indispensable for the association of E3 components with the 60S ribosomal subunit upon anisomycin treatment.

Fig. 5.

Parental HEK293T cells (A) or UFL1- (B), UFBP1- (C), and CDK5RAP3- (D) KO cells were treated with anisomycin (Ans, 0.1 μg/ml) for 30 min and then lysed. Ribosomes were separated by ultracentrifugation through sucrose density gradients. Protein samples were prepared from gradient fractions and analyzed by Western blotting using antibodies against RPL26, UFM1, UFL1, UFBP1, and CDK5RAP3.