FIG. 1.
Dbf4p levels fluctuate during the cell cycle. DBF4-proA (YCH288) cells were synchronized in G1 by addition of α-factor and released into fresh medium lacking α-factor at 25°C. Samples for FACS analysis and extract preparation were taken at the times shown. (a and b) Dbf4-ProA (a) and actin (b) were detected by immunoblotting with anti-IgG and antiactin antibodies, respectively. (c) FACS analysis of DNA content. The percentage of unbudded cells is shown on the right of the FACS analysis profile.