Fig. 3.

XCR1⁺ cDC1 are superior in the secretion of inflammatory cytokines. XCR1⁻ and XCR1⁺ cDC1 were sorted as shown in SI Appendix, Fig. S4. Subsequently, cells were stimulated or not either with 5 µg/mL R848 or 5 µg/mL pIC for 12 h. Supernatants of the cells were harvested and analyzed for the secretion of IL-12/IL-23p40, IL-12p70, IL-6, TNF, IFNλ1, IFNλ2/3, IFNα2, IFNβ, CXCL10, CCL3, CCL4, and CCL5 using the LEGENDplex Human Anti-Virus Response Panel (BioLegend). Samples were acquired using a BD LSRFortessa and data were analyzed using the LEGENDplex software suit (BioLegend). Scatter plots (black, filled circles: XCR1⁻; open circles: XCR1⁺) depict individual donors. Data for XCR1⁻ and XCR1⁺ cDC1 from the same donor are connected by line. *P < 0.05; **P < 0.01 (N = 6, paired Student’s t test).