Fig. 7.

Loss of SETD7 expression drives redistribution of FOXA1 chromatin binding. (A) Venn diagram for FOXA1 binding sites (FOXA1 ChIP-seq) in 22Rv1 cells stably expressing shNTC or shSETD7 and SETD7 binding sites in parental 22Rv1 cells. (B and C) Heatmap view (B) and average intensity curve (C) for FOXA1 binding intensity at total SETD7 sites. (D) Heatmap view for FOXA1 binding intensity at the altered FOXA1 sites (overlapped with SETD7 sites). (E) BETA to predict the association of gained FOXA1 chromatin binding sites with SETD7-regulated gene expression. (F) ChIP-qPCR of FOXA1 at three identified FOXA1-gained sites. (G) qRT-PCR for mRNA expression of these genes. (H) Graphic summary of the working model.