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The extent of 4E-BP1 dephosphorylation is proportional to the level of eIF4E expression. Parental cells (S2-6) and eIF4E-expressing cell clones C1, C2, and C3 were cultured in the absence of tetracycline for the indicated times. Total cell extract (50 μg) was immunoblotted and probed with a rabbit polyclonal anti-4E-BP1 antibody. Arrows indicate the different phosphorylation isoforms of 4E-BP1.
