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. 2023 Jul 13;42(34):2558–2577. doi: 10.1038/s41388-023-02772-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A, B Relative ABCC8 mRNA expression measured by RT-qPCR in (A) HeLa and SiHa cells and (B) HPV18+ primary keratinocytes co-transfected with E6- and E7-specific siRNA. Samples were normalised against U6 mRNA levels. Successful knockdown was confirmed by analysing E6 and E7 mRNA levels. C, D Expression levels of ABCC8 mRNA measured by RT-qPCR in (C) C33A cells and (D) NHKs transfected with GFP-tagged HPV18 oncoproteins. Samples were normalised against U6 mRNA levels and data is presented relative to the GFP-transfected control. Successful transfection was confirmed by immunofluorescence analysis (not shown). E Relative expression of ABCC8 mRNA in C33A cells stably-expressing HA-tagged HPV18 oncoproteins measured by RT-qPCR. Samples were normalised against U6 mRNA levels. Expression of oncoproteins was confirmed by western blot. F Mean DiBAC₄(3) fluorescence levels in C33A cells after transfection of FLAG-tagged HPV18 E7 and treatment with either DMSO or glibenclamide (10 μM). Samples were normalised to the pcDNA3-transfected control. G Mean DiBAC₄(3) fluorescence levels in C33A cells after co-transfection of FLAG-tagged HPV18 E7 and SUR1-specific siRNA. Samples were normalised to the pcDNA3/scramble-transfected control. H Mean DiBAC₄(3) fluorescence levels in HeLa and SiHa cells after transfection of HPV E7-specific siRNA. Samples were normalised to the scramble control. Bars represent means ± SD of three biological replicates with individual data points displayed. Ns not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (Student’s t test).