Fig. 3.

a Values are mean ± standard deviation of triplicate determinations. Crystal violet uptake (%) by bacterial strains after the treatment with < 10 kDa and < 5 kDa peptide fractions. b Inner membrane permeability of the < 10 kDa peptides fraction. Hydrolysis of ONPG due to release of cytoplasmic β-gaIactosidase in E. coli (ATCC) cells treated with peptides at 8× MIC was measured spectroscopically at an absorbance of 420 nm as a function of time. Triton-X 100 (1%) was used as a positive control, while 0.5% saline was used as a negative control