Table 2.
Primer pairs used for qPCR.
| Target feline gene | Forward (5′ → 3′) | Reverse (5′ → 3′) | Product (bp) |
|---|---|---|---|
| ACOD1 | CACTCCTGAGATAAGCCTCCTC | TCTGGCAAAGCTTTCTGTGAC | 65 |
| CLDN7 | TGAATCTGAAGTACGAGTTCGGTCC | CTCCCGGGACAGGAGCAAG | 103 |
| CXCL8 | TTTCTGCAGCTCTGTGTGAAGC | CAGTGTGGGCCACTGTCAATC | 139 |
| IL1B | GAACCAACAAGTGGTGTTCCG | TCCCGTCTTTCATCACACAGG | 122 |
| IL6 | ACACCAGTACTAACGTCCTGC | CTTCTACGGTTGGGACAGGG | 87 |
| IL10 | TCAAACAGCACGTGAACTCCC | AGGTACTCTTCACCTGCTCCAC | 123 |
| KRT78 | CAGCTCCAGAGAGAACAAGGG | GTCATTCTCAAGTGTGGCGTG | 120 |
| MMP3 | AGGACAAATACTGGCGATTTGATG | GCGAAGAGCCACTGAAGAAATAG | 150 |
| PDK4 | TTCCAGGCCAGCCAATTCAC | TCCTGGTGTTCAACTGTCGC | 103 |
| RPL13A* | ACAGAAACAAGTTGAAGTACTTGGC | CATGCCTCGCACCGTCC | 119 |
Genes, primer pairs, and product size (bp = base pairs) used for qPCR analysis. *Endogenous control gene.