Figure 3.

HCC endured high temperatures in the 3D model

(A) Images of the monolayer growth of MHCC-97H in the 2D model (upper) and the spheroids generation of MHCC-97H in the 3D model (lower) on days 1, 3, 5, and 7 (three biological replicates).

(B) Quantification of the diameters of spheroids in the 3D model on days 3, 5, and 7 (n = 5) (three biological replicates).

(C) Immunofluorescence on MHCC-97H in 2D or 3D models during the logarithmic growth phase, stained for EdU (green) and Hoechst (blue) (three biological replicates).

(D) Quantification of proliferative rates of MHCC-97H in 2D or 3D models on days 1, 3, 5, and 7 (n = 3) (three biological replicates).

(E) Cell viability and LT₅₀/LT₉₀ fitted curves of MHCC-97H in the 2D model (left) and 3D model (right), respectively, detected by trypan blue staining and live/dead staining after different heat treatments for 15 min (37–57°C) (three biological replicates).

(F) Cell viability of MHCC-97H in the 2D and 3D models after heat treatment of 51, 53, or 55°C. Mean ± standard deviation (three biological replicates). Statistical analysis, one-way ANOVA with post Tukey’s comparisons test for (B), two-tailed Student’s t test for (D), two-tailed Student’s t test with correction for multiple comparisons for (F). n.s., not significant, ∗p < 0.05, ∗∗p < 0.01.