REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-NLRP3 (for WB, IP)	Cell Signaling Technology	Cat# 15101; RRID: AB_2722591
Rabbit anti-NLRP3 (for IHC)	Affinity Biosciences	Cat# DF7438; RRID: AB_2839376
Mouse anti-NLRP3 (for IF)	AdipoGen Life Sciences	Cat# AG-20B-0014-C100; RRID: AB_2885199
Rabbit anti-ASC	Cell Signaling Technology	Cat# 67824; RRID: AB_2799736
Rabbit anti-Cleaved-IL-1β	Cell Signaling Technology	Cat# 63124; RRID: AB_2799639
Rabbit anti-IL-1β	Cell Signaling Technology	Cat# 31202; RRID: AB_2799001
Rabbit anti-Cleaved Caspase-1	Cell Signaling Technology	Cat# 89332; RRID: AB_2923067
Rabbit anti-Caspase-1	Cell Signaling Technology	Cat# 24232; RRID: AB_2890194
Rabbit anti-β-Actin	Cell Signaling Technology	Cat# 4970; RRID: AB_2223172
Rabbit anti-GAPDH	Cell Signaling Technology	Cat# 2118; RRID: AB_561053
Rabbit anti-p-p65	Abcam	Cat# ab76302; RRID: AB_1524028
Rabbit anti-p65	Abcam	Cat# ab32536; RRID: AB_776751
Rabbit anti-p-ERK	Abcam	Cat# ab201015; RRID: AB_2934088
Rabbit anti-ERK	Abcam	Cat# ab184699; RRID: AB_2802136
Rabbit anti-p-AKT	Cell Signaling Technology	Cat# 4060; RRID: AB_2315049
Mouse anti-AKT	Proteintech	Cat# 60203-2-Ig; RRID: AB_10912803
Rabbit anti-p-p38	Abcam	Cat# ab195049; RRID: AB_2576214
Rabbit anti-p38	Abcam	Cat# ab170099
Rabbit anti-PPT1	Proteintech	Cat# 29653-1-AP; RRID: AB_2923599
Rabbit anti-FLAG	Abcam	Cat# ab205606; RRID: AB_2916341
Rabbit anti-M6PR	Abcam	Cat# ab124767; RRID: AB_10974087
Rabbit anti-PSAP	Abcam	Cat# ab300469
Rabbit anti-HSC70	Proteintech	Cat# 10654-1-AP; RRID: AB_2120153
Rabbit anti-CD36	Abcam	Cat# ab252923
Rabbit anti-LRP1	Abcam	Cat# ab92544; RRID: AB_2234877
Rabbit anti-Ki-67	Abcam	Cat# ab15580; RRID: AB_443209
Rabbit anti-α-SMA	Proteintech	Cat# 14395-1-AP; RRID: AB_2223009
Rabbit anti-CD31	Proteintech	Cat# 11265-1-AP; RRID: AB_2299349
Rabbit anti-Mrc1	Servicebio	Cat# GB113497
Rabbit anti-F4/80	Servicebio	Cat# GB11027; RRID: AB_2814687
Rabbit anti-Nos	Cell Signaling Technology	Cat# 13120; RRID: AB_2687529
Rabbit anti-CD206	Cell Signaling Technology	Cat# 24595; RRID: AB_2892682
Cholera Toxin Subunit B	Thermo Fisher Scientific	Cat# C22843
Rat anti-mouse LAMP2	Proteintech	Cat# 65052-1-Ig; RRID: AB_2881468
LysoTracker red	Beyotime	Cat# C1046
LysoSensor™ Green DND-189	Yeasen	Cat# 40767ES50
APC anti-mouse CD86	Biolegend	Cat# 105012; RRID: AB_493342
PE anti-mouse CD163	Biolegend	Cat# 156703; RRID: AB_2860724
Brilliant Violet 421™ anti-mouse F4/80	Biolegend	Cat# 123137; RRID: AB_2563102
APC anti-mouse/human CD11b	Biolegend	Cat# 101212; RRID: AB_312795
APC anti-mouse CD36	Biolegend	Cat# 102611; RRID: AB_571994
TruStain FcX™ (anti-mouse CD16/32)	Biolegend	Cat# 101319; RRID: AB_1574973
ABflo™ 555-conjugated Goat Anti-Mouse IgG (H + L)	Abclonal	Cat# AS057; RRID: AB_2768321
Alexa Fluor 647-conjugated Goat Anti-Rabbit IgG (H + L)	Abclonal	Cat# AS060; RRID: AB_2768330
Fluorescein (FITC)–conjugated Goat Anti-Rat IgG(H + L)	Proteintech	Cat# SA00003-11; RRID: AB_2890989
Biological samples
Human nondiabetic wound tissue samples	the First Affiliated Hospital of Sun Yat-sen University	N/A
Human diabetic wound tissue samples	the First Affiliated Hospital of Sun Yat-sen University	N/A
Chemicals, peptides, and recombinant proteins
LPS	Sigma Aldrich	Cat# L2880
Recombinant Murine IL-4	Peprotech	Cat# 214-14
Phenylpyruvate	Sigma Aldrich	Cat# 286958
Phenylalanine	MedChemExpress	Cat# HY-N0215
Lipofectamine 2000 Transfection Reagent	Invitrogen	Cat# 11668019
Lipofectamine RNAi Max Transfection Reagent	Invitrogen	Cat# 13778030
Penicillin‒streptomycin	Sigma Aldrich	Cat# V900929
Fetal bovine serum	ThermoFisher Scientific	Cat# 10099-141
MCC950	Selleck	Cat# S8930
ATP	Selleck	Cat# S5260
Recombinant M-CSF	PeproTech	Cat# 315-02
Red cell lysis buffer	Beyotime	Cat# C3702
Palmitic acid	MedChemExpress	Cat# HY-N0830
DMEM	ThermoFisher Scientific	Cat# C11995500BT
DL-dithiothreitol	MedChemExpress	Cat# HY-15917
4-methylumbelliferyl-6-thio-βd-gluco-pyranoside	Medbio	Cat# MED20989
β-glucosidase	Sigma Aldrich	Cat# G4511
Critical commercial assays
IP-ABE Palmitoylation Kit	AIMS	Cat# AM101314
HiScript 1st Strand cDNA Synthesis Kit	Vazyme, China	Cat# R111
Real-time Fluorescent Quantitative PCR Kit	Vazyme, China	Cat# Q321
ECL detection reagent	EpiZyme	Cat# SQ202
BCA assay kit	Thermo Fisher Scientific	Cat# 23225
IL-1β ELISA KIT	MEIMIAN	Cat# MM-0040M2
IL-18 ELISA KIT	MEIMIAN	Cat# MM-0169M2
TNF-α ELISA KIT	MEIMIAN	Cat# MM-0132M2
Deposited data
LC-MS raw data files	https://www.ebi.ac.uk/metabolights/	MTBLS7853
See Table S3 for LiP-SMap determination of PPA-bound proteins	This paper	N/A
Experimental models: Cell lines
Murine BMDM	This paper	N/A
Mouse embryonic fibroblasts	This paper	N/A
Experimental models: Organisms/strains
C57BL/6J mice	GemPharmatech	N/A
BKS-DB mice	GemPharmatech	N/A
Oligonucleotides
Primers for qRT-PCR, see Table S4	Ruibiotech	N/A
siRNA, see Table S5	GenePharma	N/A
Recombinant DNA
pIRES2-Flag-Ppt1	Umine Biotechnology	N/A
pIRES2-Flag-Ppt1-MUT	Umine Biotechnology	N/A
Software and algorithms
FlowJo software Version 10.8.1	BD Biosciences	https://www.flowjo.com/
ImageJ	National Institutes of Health (NIH)	https://imagej.nih.gov/ij/
GraphPad Prism 8	GraphPad Software	https://www.graphpad.com/scientificsoftware/prism/
Autodock	Autodock Software	https://autodock.scripps.edu/
Adobe Illustrator	Adobe	https://www.adobe.com/
Other
Phenylalanine diets, see Table S2	This paper	N/A