Table 4.

IC50 of the 18 bark extracts to inhibit egg hatching, ordered from the lowest to the highest concentration against two ovine GIN species.

T. colubriformis				T. circumcincta
Bark	Season	Solvent	IC50 (µg DM/mL)	Bark	Season	Solvent	IC50 (µg DM/mL)
P	W	Ace-Wa	48	P	W	Ace-Wa	221
P	W	Met-Wa	63	S2	W	Ace-Wa	221
P	S	Ace-Wa	89	S1	W	Ace-Wa	353
P	S	Met-Wa	93	S2	W	Met-Wa	407
S2	W	Ace-Wa	95	P	W	Met-Wa	407
S2	W	Met-Wa	145	P	W	Wa	459
S2	S	Ace-Wa	159	S1	W	Met-Wa	475
S1	W	Ace-Wa	162	P	S	Ace-Wa	492
S2	S	Met-Wa	193	P	S	Met-Wa	596
S1	S	Met-Wa	196	S1	S	Ace-Wa	1050
S1	W	Met-Wa	208	S2	W	Wa	1282
S1	S	Ace-Wa	400	P	S	Wa	3587
P	W	Wa	495	S2	S	Ace-Wa	5908
S2	W	Wa	856	S1	W	Wa	7093
P	S	Wa	3,498	S2	S	Met-Wa	20,733
S1	W	Wa	16,203	S1	S	Met-Wa	83,393
S1	S	Wa	212,179	S1	S	Wa	4,236,058
S2	S	Wa	1.814	S2	S	Wa	1.211

IC50: lethality dose required to inhibit 50% of the GIN eggs from hatching. Calculated based on results from dose–response egg hatch assay (EHA). Bark extracts tested included: (S1): spruce, sawmill, ring debarking; (S2): spruce, pulp mill, drum debarking; (P): pine, sawmill, ring debarking and were collected during summer (S) and winter (W) seasons. Each of the barks were extracted using water (W), acetone–water (Ace-Wa), and methanol–water (Met-Wa) as the solvent.