REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-human CD154-FITC (clone: TRAP1)	BD Biosciences	cat# 555699; RRID: AB_396049
Mouse anti-human CD3-BUV615 (clone: SP34-2)	BD Biosciences	cat# 751249; RRID: AB_2875266
Mouse anti-human CD4-PE-Cy5.5 (clone: S3.5)	Thermo Ficher Scientific	cat# MHCD0418; RRID: AB_10376013
Mouse anti-human CD8-BUV563 (clone: RPA-T8)	BD Biosciences	cat# 612914; RRID: AB_2870200
Mouse anti-human CD27-PE-Cy5 (clone: 1A4CD27)	Beckman coulter	cat# 6607107
Mouse anti-human CD45RO-BUV805 (clone: UCHL1)	BD Biosciences	cat# 748367; RRID: AB_2872786
Mouse anti-human IFN-g-BV786 (clone: 4S.B3)	BioLegend	cat# 502542; RRID: AB_2563882
Mouse anti-human TNF-BV650 (clone: MAb11)	BioLegend	cat# 502938; RRID: AB_2562741
Rat anti-human IL-13-BV421 (clone: JES10-5A2)	BD Biosciences	cat# 563580; RRID: AB_2738290
Mouse anti-human IL-21-Ax647 (clone: 3A3-N21)	BD Biosciences	cat# 560493; RRID: AB_1645421
Mouse anti-human IL-4-PE-Cy7 (clone:8D4-8)	BD Biosciences	cat# 560672; RRID: AB_1727547
Mouse anti-human IL-17A-BV605 (clone: BL168)	BioLegend	cat# 512326; RRID: AB_2563887
Rat anti-human IL-2-BUV737 (clone: MQ1-17H12)	BD Biosciences	cat# 612836
Mouse anti-human CD107A-BV711 (clone: H4A3)	BioLegend	cat# 328640; RRID: AB_2565840
Mouse anti-human MIP1b-Alexa700 (clone: D21-1351)	BD Biosciences	cat# 561278; RRID: AB_10612008
Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG1 (AS35)	ACROBiosystems	cat# SAD-S35
Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG2 (AS35)	ACROBiosystems	cat# SAD-S66
Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG3 (AS35)	ACROBiosystems	cat# SAD-S67
Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG4 (AS35)	ACROBiosystems	cat# SAD-S68
Mouse anti-Human IgG1 Fc Secondary Antibody, HRP	Thermo Fisher Scientific	cat# MH1715; RRID: AB_2539710
Mouse Anti-Human IgG2 Fc-BIOT HP6002	SouthernBiotech	cat# 9070-08; RRID: AB_2796638
Mouse Anti-Human IgG3 Hinge-BIOT HP6050	SouthernBiotech	cat# 9210-08; RRID: AB_2796700
Mouse Anti-Human IgG4 Fc-BIOT HP6025	SouthernBiotech	cat# 9200-08; RRID: AB_2796692
Biological samples
Human PBMCs	This study	This study
Chemicals, peptides, and recombinant proteins
Benzonase Nuclease, Purity >90%	MERCK Millipore	cat# 70746
BD GolgiPlug	BD Biosciences	cat# 555029
BD GolgiStop	BD Biosciences	cat# 554724
LIVE/DEADTM Fixable Blue Dead Cell Stain Kit	Thermo Fisher Scientific	cat# L23105
Cytofix/Cytoperm kit	BD Biosciences	cat# 554714
RBD protein	SinoBiological	cat# 40592-VNAH
Peroxidase Substrate Solution B	KPL	cat# 50-65-02
TMB Peroxidase Substrate	KPL	cat# 50-76-02
HRP-conjugated streptavidin	Thermo Fisher Scientific	cat# N100
Peptide sequence for T cell analysis (See Table S5)	This paper	N/A
TransIT-LT1	Mirus Bio	MIR2300
pCAGG -SARS-CoV-2-Wuhan spike	This paper	N/A
pCAGG -SARS-CoV-2- Delta spike	This paper	N/A
pCAGG -SARS-CoV-2- Omicron BA.2 spike	This paper	N/A
VSVΔG-Luc/G	Matsuura et al.31	N/A
VSVΔG-Luc/SARS-CoV-2 Wuhan spike	This paper	N/A
VSVΔG-Luc/SARS-CoV-2 Delta spike	This paper	N/A
VSVΔG-Luc/SARS-CoV-2 Omicron BA.2 spike	This paper	N/A
Luciferase assay system	Promega	E1500
Critical commercial assays
V-PLEX SARS-CoV-2 Panel 7 (ACE2) multiplexed immunoassay kit	Meso Scale Diagnostics	Cat# K15440U-2 and K15440U-4
Deposited data
SARS-COV-2 Wuhan signal sequence	GenBank	YP_009724390.1 amino acids [aa] 1–13 of S
SARS-COV-2 Wuhan RBD sequence	GenBank	YP_009724390.1 amino acids [aa] 327–531 of S
Influenza hemagglutinin (HA) sequence	GenBank	P03452.2 [aa] 518-565
SARS-CoV-2 Wuhan spike gene	GenBank	NC_045512.2
SARS-CoV-2 Delta spike gene	GenBank	QWA53965.1
SARS-CoV-2 Omicron spike gene	GenBank	UFO69279.1
Experimental models: Cell lines
HEK239T cells	ATCC	CRL-3216
Vero cells	ATCC	CCL-81
Software and algorithms
Discovery Workbench software 4.0	Meso Scale Diagnostics	https://www.mesoscale.com/en/products_and_services/software
FlowJo 10.7.1	BD Biosciences	https://www.flowjo.com/
GraphPadPrism version 8	GraphPad Software, Inc.	https://www.graphpad.com
GraphPadPrism version 9	GraphPad Software, Inc.	https://www.graphpad.com