REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Biological samples | ||
cervical scrapings | Xiangya Hospital of Central South University, the Second Xiangya Hospital of Central South University, the Third Xiangya Hospital of Central South University, the First Affiliated Hospital of Shantou University Medical College, Loudi Central Hospital and Zhengzhou Central Hospital Affiliated to Zhengzhou University | This study |
Critical commercial assays | ||
Human Papillomavirus DNA (Genotype) Diagnostic Kit | SANSURE | S3027E |
EZ DNA Methylation-Direct ™ Kit | ZYMO RESEARCH | D5021 |
HiPure Universal DNA Kit | Magen Biotech | D3018-02 |
Uterine cervix cancer gene methylating Diagnostic Kit(PCR-Fluorescence Probing) | HOOMYA | HOOMYA |
Oligonucleotides | ||
ZNF671-F: GGTGGAGGTGTTGGGAAA | This paper | N/A |
ZNF671-R: 5′- CTAAAACACAAAAACTA CAAACACTTTAC-3′ |
This paper | N/A |
ZNF671-S: 5′- GTTTGATGTTTTGT AGGGA-3′ |
This paper | N/A |
ZNF671-QF:5′- GTCGTTTTCGGTA GTTGTTCGC -3′ |
This paper | N/A |
ZNF671-QR: 5′- AAAAACGCAACAC CCACCC -3′ |
This paper | N/A |
ZNF671-P: 5′-FAM- TTTACGGTTTTA TGGCGGA-MGB-3′ |
This paper | N/A |
β-actin-QF: 5′-AGGAGGTTTGGATTTT TAATTGTGTATAG-3′ |
This paper | N/A |
β-actin-QR:5′-AACTCACCACTACAAAA ATCAAACCA -3′ |
This paper | N/A |
β-actin-P:5′-VIC-TGGTAGAGAGGAAG GTGG-MGB-3′ |
This paper | N/A |
Software and algorithms | ||
SPSS software | SPSS Inc | version 22.0 |
R | R-project | version 3.6.3 |