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. 2023 Jun 7;7(5):100200. doi: 10.1016/j.rpth.2023.100200

Figure 4.

Figure 4

Platelet factor XIII-A inhibition or absence reduces platelet thrombus formation on fibrinogen but not collagen. (A, B) Whole-blood platelet thrombus formation under flow was performed with healthy donor blood at 500/s in capillary chambers coated with 100 μg/mL of fibrinogen with added DiOC6 (4 μg/mL) to label platelets and recorded over 600 seconds (s) using fluorescence confocal microscopy. (A) Data represents fluorescence intensity (FU) ± SEM of platelet deposition in the absence (blue) and presence (gray) of transglutaminase inhibitor (TGI) for over 600 seconds. Statistical analysis was performed on endpoint thrombus formation at the 600-second time point and compared in samples with and without TGI using paired t-test, ∗P < .05. n = 3. (B) Representative images of endpoint thrombus formation at the 600-second time point in healthy donor blood in the absence (left) and presence (right) of TGI. Scale bar represents 20 μm. (C) Platelet thrombus formation was performed at 1000/s on 100 μg/mL of collagen with whole blood from normal healthy donors (blue; left panel) and factor XIII (FXIII)–deficient (FXIII-def) donors (green; left panel); whole blood from normal healthy donors was also assayed in the absence (blue; right panel) and presence (black; right panel) of TGI for over 600 seconds. Data represent mean FU ± SEM. Statistical analysis was performed on endpoint thrombus formation at the 600-second time point and compared in samples with and without TGI using the paired t-test. Normal donors: n = 3; FXIII-deficient donors: n = 2. (D) Representative images of endpoint thrombus formation at the 600-second time point in FXIII-deficient patient blood (left) and healthy donor blood in the absence (middle) and presence (right) of TGI. Scale bar represents 20 μm. ns, nonsignificant.