Table 2.
System Suitability parameters of the proposed HPLC–DAD method
| Parameter | ATN | AMI | DSA | CT | HCT | AML | VAL | CAN | Reference value [59] |
|---|---|---|---|---|---|---|---|---|---|
| Selectivity (α) | 1.37 | 1.70 | 1.11 | 1.07 | 1.24 | 1.11 | 1.25 | α > 1 | |
| Resolution (Rs) | 2.86 | 7.43 | 2.80 | 2.50 | 9.00 | 4.00 | 7.43 | Rs > 1.5 | |
| Tailing factor (T) | 1.15 | 1.23 | 0.95 | 1.06 | 0.97 | 1.07 | 0.98 | 1.14 | T ≤ 2, T = 1 for symmetric peak |
| Column efficiency (N) | 4806 | 3844 | 13766 | 36100 | 40000 | 55695 | 29122 | 23716 | N > 2000 |
| Height equivalent to theoretical plate (HETP) (cm/plate) | 0.0052 | 0.0065 | 0.0018 | 0.0007 | 0.0006 | 0.0004 | 0.0009 | 0.0010 | The smaller the value, the higher column efficiency |
| Retention time (TR) (min ± 0.2) | 5.21 | 6.16 | 8.82 | 9.51 | 10.04 | 11.89 | 12.84 | 15.02 |