Fig. 7.

HIF-1A drives the downregulation of PUS10 in a transcriptional manner. A, B qRT‒PCR and western blotting showed that the expression of PUS10 was repressed at the mRNA and protein levels under hypoxia in Caki-1 and OS-RC-2 cell lines. C, D qRT‒PCR and western blotting showed that silencing HIF-1A but not HIF-2A increased the repression of PUS10 expression under hypoxia. E Online data from the Clinical Proteomic Tumor Analysis Consortium identified the accumulation of HIF-1A in KIRC. F Chromatin immunoprecipitation (ChIP) was performed to determine the binding between predicted binding sites on promoters and HIFs. G Dual luciferase reporter assays were performed by transfecting plasmids containing the WT or Mut promoter region of NUDC and siRNA targeting HIFs into HEK-293T cells. The relative luciferase activity was normalized to Renilla luciferase activity. Data from three independent experiments are presented as the mean ± S.D. *P < 0.05, **P < 0.01, ***P < 0.001; ns not significant