Table 4.

Effects of crude protein level and sanitary conditions on biogenic amine concentrations in jejunal and colonic digesta in weaned pigs¹

	Clean conditions			Unclean conditions			SEM	P-values2
Item, mg/g	HCP	LCP 1	LCP 2	HCP	LCP 1	LCP 2		San	CP	AA	San × CP
Jejunum
Putrescine	0.10	0.06	0.05	0.12	0.09	0.06	0.031	0.420	0.112	0.554	0.935
Histamine	0.05	0.12	0.04	0.08	0.12	0.03	0.020	0.608	0.419	0.001	0.360
Cadaverine	0.41	0.39	0.41	0.34	0.51	0.43	0.128	0.833	0.571	0.796	0.493
Spermidine	0.10	0.08	0.06	0.09	0.10	0.13	0.023	0.176	0.872	0.780	0.152
Tyramine	0.03	0.02	0.00	0.01	0.03	0.03	0.017	0.447	0.922	0.786	0.158
Spermine	0.05	0.05	0.07	0.04	0.05	0.06	0.009	0.528	0.274	0.291	0.637
Tryptamine	0.01	0.01	0.02	0.01	0.02	0.02	0.004	0.395	0.067	0.851	0.402
Colon
Putrescine	0.13	0.14	0.14	0.14	0.19	0.22	0.034	0.135	0.170	0.732	0.383
Histamine	0.04	0.07	0.04	0.02	0.11	0.05	0.017	0.339	0.026	0.008	0.112
Cadaverine	0.81	1.09	1.31	0.65	1.45	0.97	0.218	0.784	0.016	0.564	0.651
Spermidine	0.29ab	0.24ab	0.20b	0.31ab	0.37a	0.39a	0.035	0.001	0.952	0.774	0.031
Tyramine	0.09	0.05	0.02	0.04	0.05	0.03	0.027	0.522	0.300	0.306	0.210
Spermine	0.03bc	0.03c	0.03c	0.04bc	0.05ab	0.06a	0.005	0.001	0.085	0.221	0.007
Tryptamine	0.03	0.04	0.03	0.03	0.04	0.03	0.005	0.675	0.240	0.005	0.554

¹HCP, high crude protein; LCP 1, low crude protein supplemented with nine indispensable crystalline amino acids except for Arg; LCP 2, low crude protein supplemented with only six indispensable crystalline amino acids (Lys, Met, Thr, Trp, Val, and Ile).

²San, main effect of sanitation; CP, main effect of crude protein levels (HCP vs. LCP 1 and LCP 2); AA, main effect of crystalline amino acids supplementation patterns (LCP 1 vs. LCP 2); San × CP, interactive effect of sanitation and crude protein levels (HCP vs. LCP 1 and LCP 2).

^a,bWithin a row, means with different superscripts differ (P < 0.05).