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. Author manuscript; available in PMC: 2023 Aug 19.
Published in final edited form as: J Am Chem Soc. 2023 Jun 23;145(26):14208–14214. doi: 10.1021/jacs.3c04047

Figure 4.

Figure 4.

(a) Analytical ultracentrifugation profiles of apo-TP1 (blue) and Zn-TP1 (magenta). (b) Thermal denaturation of apo-TP1 (blue), Zn-TP1 (magenta) and ApoCyt (grey). (c) Superposition of experimental (magenta) and designed (grey) structures of Zn-TP1 (PDB: 8SJH), and (d) close-up views of engineered interfacial residues. (e) Views of the Zn-66His3 center (Zn – grey sphere, chloride – green sphere), along with 2Fo-Fc (grey mesh, 1.0σ) and Zn-anomalous maps (blue mesh, 5.0σ). (f) Superposition of Zn-TP1 (magenta) and apo-TP1 (cyan, PDB: 8SJI) structures.