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(a) Orientation of ApoCyt monomers in ZnII-Tet4. (b) Orientation of ApoCyt monomers (left) and the central water channel in Zn-TP1. (c) The investigated hydride transfer reaction (top) and corresponding chiral-HPLC traces of relevant species. Reaction conditions were: 10 μmol substrate, 30 μmol phenylsilane, 0.01 μmol protein trimer and/or ZnCl2, incubated for 6 h at 20 °C.
