Fig. 1: Single-cell transcriptomic landscape of NRAS-driven melanoma.

a, Uniform Manifold Approximation and Projection (UMAP) of malignant cells analysed by scRNA-seq and integrated across five different NRAS^Q61K/°;Ink4a^−/− primary lesions. b, Mitf regulon activity in mouse malignant cells projected as UMAP (upper panel) and violin plot (lower panel), respectively. The middle line represents the median. c. Functional enrichment terms of the characteristic Seurat clusters identified in a (Fisher exact test, two-tailed p value). d. Heatmap illustrating discriminative marker genes (n=9) for each functionally enriched state. e. AUCell score of top functionally enriched marker genes per state projected on malignant UMAP (see Supplementary Table 1). f, Mapping of gene expression program activities during murine neural crest development¹⁸ onto transcriptional states of NRAS^Q61K/°Ink4a^−/− melanoma. g, Activity (binary AUCell score) of selected mouse malignant gene signatures from NRAS^Q61K/°;Ink4a^−/− melanoma projected onto scRNAseq datasets from BRAF^V600E;Pten^l/l mouse melanoma lesions (left panel) and human clinical Metastatic Melanoma samples²² (right panel).