Extended Data Fig. 5: Hierarchical melanoma growth is independent of the adaptive immune system.

a, Schematic representation of the generated melanoma mouse model transplanted in immunocompromised mice (Foxn1^nu). Schematic made with Biorender.com. b, Tumour growth kinetics (mm³) of individual tumours in immunodeficient mice (yellow), and corrected mean obtained from fitting an exponential growth to each individual sample (black curve). Dashed lines indicate tumour duplication times. Dotted red curve refers to the mean growth kinetics extracted from Fig. 2b. c, Confocal images showing Confetti labelling efficiency in melanoma tumours 3 days after the administration of different doses of TAM in order to achieve the optimal dose to perform clonal analyses. Representative images from n=3 independent tumors. d, Confocal image showing Confetti labelling of melanoma cells 5 days after TAM administration (0.5 mg) in immunodeficient background. Representative image from n=5 independent tumors. e, Violin plot depicting the proportion of labelled melanoma cells expressing each Confetti fluorophore 5 days after TAM administration (0.5 mg). Six melanoma tumours (two sectional area of each) were analysed. Black line represents median and grey lines the 25^th to 75^th percentile. f, Confocal images of melanoma tumours in immunodeficient background exhibiting Confetti labelling 5 days after initial induction (1.5-fold tumour increase) and at relative growth of 10-fold upon single low dose intraperitoneal Tamoxifen administration (0.5 mg). Representative images from n=3 independent tumors.