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. 1999 Jun;19(6):4390–4404. doi: 10.1128/mcb.19.6.4390

FIG. 1.

FIG. 1

FIG. 1

FIG. 1

BP-1 interacts with E1B 19K in yeast. (A) The yeast two-hybrid assay was used to demonstrate binding between BP-1 and E1B 19K. Growth in the presence of histidine indicates that both plasmids can be expressed in yeast, and growth in the absence of histidine demonstrates an interaction between the two proteins. Apc-2 represents an irrelevant hydrophobic protein used as a negative control. The specificity of the interaction between BP-1 and E1B 19K was tested by using related proteins (Bcl-2, Bcl-xL, and Bax) as well as missense mutants of E1B 19K (pm7, pm51, pm87, and pm102). (B) The minimal regions of E1B 19K required for interaction with BP-1 were mapped by using a series of deletion mutants. (C) Schematic representation of E1B 19K showing the missense and deletion mutants tested in the two-hybrid assay. Regions I and III indicate the locations of the Bcl-2 homology domains BH1 and BH3, respectively. E1B 19K does not contain recognizable BH2 or BH4 domains.