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BtfS binds to DNA-cellulose in vitro. [35S]methione-labeled, in vitro-translated Myc-BtfS and E1B 19K were prepared by using the TnT T7 reticulocyte lysate system and incubated with native DNA-cellulose in NETN buffer for 2 h. Samples were washed five times in NETN, and the proteins were resolved by SDS-PAGE (17% polyacrylamide). Immunoprecipitated proteins (lanes 1 and 2) were analyzed to confirm the presence of the in vitro-translated products.
