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BtfS inhibits transformation by E1A and E1B 19K (A) or p53DD (B). Primary BRK cells were transfected with carrier DNA along with a linearized test DNA (15 μg of pCMV-E1A, 15 μg of pCMV-E1B 19K or pCMV-p53DD, and 45 μg of pcDNA3-Myc-BtfS). DNA concentrations were kept constant by using appropriate empty vectors. The cells were cultured for 3 to 4 weeks and then stained with Giemsa. Foci were counted from four dishes per condition. Bars indicate standard deviations (n = 4).
